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Cat No | BSQ-006 |
Conjugate | |
Type | Protein extraction and quantification |
Source | N/A |
Size | 1ml×10 |
Application | Inhib |
Format | Liquid |
Concentration | Please refer to the vial lable for the specific concentration. |
Storage | Store at -20℃ stable for at least 12 months. |
Synonyms | Protease inhibitor cocktail |
Purification | |
Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
MolecularWeight | |
Description | 哺乳动物细胞或组织提取物中含有许多内源性的蛋白酶, 磷酸酶等, 容易导致提取物中的蛋白降解或去修饰, 从而影响后续的蛋白检测. 因此在提取物中添加适当的蛋白酶, 磷酸酶等抑制剂是防止蛋白降解和去修饰的有效方法. Bioss生产的蛋白酶抑制剂混合物(哺乳动物样品抽提用, 100×) (Protease inhibitor cocktail for mammalian cell and tissue extracts, 100×) 是一种用于哺乳动物细胞或组织蛋白提取的蛋白酶抑制剂混合物, 主要成分有:105mM AEBSF, 80uM Aprotinin, 5mM Bestatin, 1.5mM E64, 2mM Leupeptin and 1.5mM Pepstatin A in DMSO, 并提供一支独立包装的0.5M EDTA, pH 8.0, 以便选择性使用. 注:如果所提取的蛋白质用于检测金属蛋白酶活性, 则不宜添加EDTA. 本产品为100×浓缩液, 使用时请按1:100的比例加入裂解液中, 用于哺乳动物细胞或组织蛋白的提取, 可有效抑制蛋白降解. 该产品可以抑制绝大多数蛋白酶活性, 包括丝氨酸蛋白酶, 半胱胺酸蛋白酶, 胃蛋白酶, 胰蛋白酶, 木瓜蛋白酶等. 所提取的蛋白质可以用于 Western Blot, 免疫共沉淀等试验. |
Background | They can inhibit the activity of trypsin, papain, chymotrypsin, elastase, pepsin, metalloproteinase and other proteases, thus effectively inhibiting the degradation of protein, and maintaining the original protein structure. |