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胶体金标记技术服务

服务编号:BIOFW002


胶体金是由氯金酸(HAuCl4)在还原剂如柠檬酸钠等作用下,聚合成一定大小的金颗粒,并由于静电作用成为一种稳定的胶体状态,形成带负电的疏水胶溶液,由于静电作用而成为稳定的胶体状态,故称胶体金。胶体金在弱碱环境下带负电荷,可与蛋白质分子的正电荷基团形成牢固的结合,由于这种结合是静电结合,所以不影响蛋白质的生物特性。

 

胶体金标记是蛋白质等高分子被吸附到胶体金颗粒表面的包被过程。吸附机理可能是胶体金颗粒表面负电荷,与蛋白质的正电荷基团因静电吸附而形成牢固结合。也可以通过化学结合蛋白。

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Passive Absorption

Popular and simple

Cannot provide a permanent attachment of the coating

The proteins may lose their properties after being absorbed to the surface

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Covalent Coupling

Permanently immobilizes molecules of interest to functionalized gold nanoparticles

More specific and controllable

Minimizing steric hindrance and impact on the tertiary structure of the conjugated protein

 

免疫金标记技术主要利用金颗粒具有高电子密度的特性,当这些标记物在相应的配体处大量聚集时,肉眼可见红色或粉红色斑点,因而用于定性或半定量的快速免疫检测方法中。

 

北京百欧泰生物科技有限公司还可以提供金纳米颗粒、金纳米棒、银纳米颗粒等纳米材料产品。

 

Our Available Gold Nanoparticles Conjugates

Biotin Gold Nanoparticles

Protein A Gold Nanoparticles

Protein G Gold Nanoparticles

Transferrin Gold Nanoparticles

Streptavidin Gold Nanoparticles

Anti-Goat Gold Nanoparticles

Anti-Sheep Gold Nanoparticles

Anti-Human Gold Nanoparticles

Anti-Mouse Gold Nanoparticles

Anti-Rabbit Gold Nanoparticles 

Anti-Rat Gold Nanoparticles

 

产品特点

Stable

Well Characterized

Customer can select buffer

Customer can select gold nanoparticle type, size

Quick turnaround

 

备注可按照要求定制请联系客服qq 499854788

 

Publications

Cat No

Product Name

Publications

BIOSV005

Cy标记大分子蛋白/抗体

Lian, Z., Wu, T., Wang, H., Chi, J., Cheng, L., Xie, D., Pan, X., Hu, Y., Tan, Z., Chen, S., Yang, X., Yun, Y., Wu, W., Li, C., Su, M., Song, Y., At-Home COVID-19 Rapid Antigen Test Down to 0.03 pg mL?1 of Nucleocapsid Protein. Small 2023, 19, 2301162. https://doi.org/10.1002/smll.202301162

BIOSV001

FITC抗体标记技术服务

Jimei C, Yonggan X, Yinying Z, Teng H, Bobin N, Lijun C, Hongfei X, Huadong W, Wenchen W, Qingyu Meng, Kaijie F, Fangming G, Junzhen F,  Nan J, Zheng L, Ke P, Hongyu S, Jiajin Z, Qian Z, Jiandong W, Meng S*,and Yanlin S. Perovskite Probe-Based Machine Learning Imaging Model for Rapid Pathologic Diagnosis of Cancers. ACS Nano 2024,  18,  35,  24295-24305.



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