胶体金是由氯金酸(HAuCl4)在还原剂如柠檬酸钠等作用下,聚合成一定大小的金颗粒,并由于静电作用成为一种稳定的胶体状态,形成带负电的疏水胶溶液,由于静电作用而成为稳定的胶体状态,故称胶体金。胶体金在弱碱环境下带负电荷,可与蛋白质分子的正电荷基团形成牢固的结合,由于这种结合是静电结合,所以不影响蛋白质的生物特性。
胶体金标记是蛋白质等高分子被吸附到胶体金颗粒表面的包被过程。吸附机理可能是胶体金颗粒表面负电荷,与蛋白质的正电荷基团因静电吸附而形成牢固结合。也可以通过化学结合蛋白。
Passive Absorption
Popular and simple
Cannot provide a permanent attachment of the coating
The proteins may lose their properties after being absorbed to the surface
Covalent Coupling
Permanently immobilizes molecules of interest to functionalized gold nanoparticles
More specific and controllable
Minimizing steric hindrance and impact on the tertiary structure of the conjugated protein
免疫金标记技术主要利用金颗粒具有高电子密度的特性,当这些标记物在相应的配体处大量聚集时,肉眼可见红色或粉红色斑点,因而用于定性或半定量的快速免疫检测方法中。
北京百欧泰生物科技有限公司还可以提供金纳米颗粒、金纳米棒、银纳米颗粒等纳米材料产品。
Our Available Gold Nanoparticles Conjugates
Biotin Gold Nanoparticles
Protein A Gold Nanoparticles
Protein G Gold Nanoparticles
Transferrin Gold Nanoparticles
Streptavidin Gold Nanoparticles
Anti-Goat Gold Nanoparticles
Anti-Sheep Gold Nanoparticles
Anti-Human Gold Nanoparticles
Anti-Mouse Gold Nanoparticles
Anti-Rabbit Gold Nanoparticles
Anti-Rat Gold Nanoparticles
产品特点
Stable
Well Characterized
Customer can select buffer
Customer can select gold nanoparticle type, size
Quick turnaround
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