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其他试剂盒

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F(ab)2 制备试剂盒

Cat NoBIOK2023
Type制备试剂盒
SourceN/A
Size10 Antibody Samples
Application

Fragmentation

StorageStore at 2-8℃.
SynonymsF(ab)2 fragments Micro Preparation Kit;F(ab)2 Preparation Kit;F(ab)2 微型制备试剂盒;F(ab)2 制备试剂盒;Fragmentation;F(ab)2 制备试剂盒;Fab 制备试剂盒;Fab2 制备试剂盒;抗体片段试剂盒
NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
BackgroundF(ab)2 Preparation Kit enables efficient generation of F(ab)2 from IgG. This kit uses Immobilized Pepsin, a nonspecific endopeptidase that is active only at acid pH and irreversibly denatured at neutral or alkaline pH. Pepsin digestion typically produces a F(ab)2 fragment (~110kDa by SDS-PAGE under non-reducing conditions) and numerous small peptides of the Fc portion. The resulting F(ab)2 fragment is composed of a pair of Fab units connected by two disulfide bonds. The Fc fragment is extensively degraded and can be separated from F(ab)2 by dialysis, gel filtration or ion exchange chromatography.
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